Therapeutic potential of quercetin in rat offspring cerebellum and testis of father subjected to Cisplatin treatment

There is no research published in literature concerning the dramatic changes in the offspring of fathers treated with cisplatin. The present research focused on toxicity of cisplatin on cerebellum and testis of offspring that are paternally subjected to cisplatin treatment and therapeutic potential of quercetin. Thirty -two male and female rats weighing approximately 150 gram body weight were used. They were classified into four groups (n=8); control, quercetin, cisplatin, and cisplatin and quercetin. Males were only treated and females were used only for mating and investigated their newly progeny for histopathological abnormalities in testis and cerebellum. Offspring paternally treated with cisplatin exhibited deformation of seminiferous tubules associated with massive degeneration of gonocytes. Widespread of cellular debris was detected within the lumina of the tubules. Early sign of sclerotic tubules were ensheathed by dense aggregation of fibroblast cells around the tubules. Also, the treated cerebellar cortex developed different grades of neurotoxicity. Vacuolar degeneration and fragility are common in both external granular and molecular layer. The internal granular cells were sparsely widely separated and angiogenesis was detected in between the cerebellar folia.  Hypoplasia of cerebellar cortex was clarified by reduced migration of granular cell to the inner one. Neuronal cell death was measured by increased dark-brown caspase 3 immunohistochemistry. The mentioned drastic effects were improved in offspring testis and brain paternally received co-administration of quercetin plus cisplatin-treatment. The improvement of both organs was attributed to the quercetin antioxidant activity plus its  cytological inclusion of micromolecules which activate cell defense.