The Breeding of Transgenic Livestock New Lines

The integration and expression of foreign genes in the G0 transgenic livestock's which obtained by using existed transgenic technology were first analysized.There were a lot of chimerism in the cases of random integration,and the expression effects were also different in different integration sites.It should be relatively simple if targeted integration was achieved by gene targeting technology.On this basis,the methods and procedures of the selection for G0 transgenic animals in different integration types were proposed,and two technical routes for homozygous transgenic animals were established.The first one was to select genetic stable G0 individuals and obtain homozygote through conventional breeding.The second one was to repeatly use somatic cell gene targeting to obtain the livestock with dual-allele interaction(or knockout).Finally,according to the livestock breeding theory,the proncess of constructing a new trangenic line and then forming a new species based on the transgenic homozygous individuals was put forward.