Comparative analysis of genomic DNA isolation procedures; hexa decyltrimethylammonium bromide (CTAB) and liquid detergent (morning fresh ® ) methods from Samsorg 41 (icsv 400)

Genomic DNA was prepared from germinating seeds of a variety of sorghum in Nigeria (Samsorg 41) using two different isolation protocols (CTAB and morning fresh® method). Highest quantity of DNA was obtained from Samsorg 41 using the morning fresh® method. Genomic DNA obtained using this method had a much lower purity as compared with that obtained using the CTAB method. Both methods produced genomic DNA of high integrity with minimum shearing.   Key words: Genomic DNA, CTAB, morning fresh®, isolation, Samsorg 41, Nigeria.