Classical swine fever virus-Shimen infection upregulates SH3GLB1 expression in porcine alveolar macrophages

AbstractAlthough SH3GLB1 is well known as an autophagy-related protein, its response mechanism to virus infection has yet to be determined. Through digital gene expression (DGE) profile analysis, we identified that SH3GLB1 may be a key gene responding to classical swine fever virus (CSFV) infection in porcine alveolar macrophages (PAMs). The present study found that the mRNA expression and the SH3GLB1 protein level in CSFV Shimen infected PAMs were significantly higher than those of mock-infected PAMs. Furthermore, confocal microscopy analysis suggested that SH3GLB1 was temporally co-localised with CSFV E2. These findings contributed in developing our understanding of the mechanism of CSFV infection.