DEVELOPMENT OF RP-HPLC METHOD FOR METFORMIN AND REPAGLINIDE IN RABBIT PLASMA

A sensitive, specific and rapid high-performance liquid chromatography-ultraviolet spectroscopy method was developed and successfully validated to estimate the metformin and repaglinide in rabbit plasma. The solvent extraction method was used for metformin and repaglinide from serum by using ethyl acetate and 0.1N HCl. The mobile phase consists of acetonitrile: phosphate buffer pH 4.0 at 60:40 %v/v with 1% triethylamine at flow rate of 0.8ml/min and at fixed wavelength of 254nm. On ten minutes of run time, metformin was retention at 5.1 and repaglinide for 7.4min. The extraction efficiency was 98% for metformin and 95% for repaglinide. The intra-day and inter-day precision was in the terms of %RSD less than 1.76% for both the compounds. The developed method was validated and proposed method is useful for pharmacokinetics studies.