DSRG Metagenomics Study: Next-Generation Techniques and Data Analysis

Next-generation sequencing has vastly changed the processes associated with metagenomic research and there is a current rush of scientific studies in this field. However, although much has been published, there are still many unanswered questions. In this DSRG study we aim to answer some of the larger questions by evaluating different DNA extraction methods and comparing two platforms, the Roche/454 and the Illumina next-generation systems. Here, we present initial results from two community samples using one extraction method. We examined both 16S and whole genome DNA samples from each and sequenced all samples on the two next-generation platforms. We detail the different analysis methods used to evaluate the sequence data off these platforms and show how the platforms perform comparatively. Future goals of the study will also be highlighted with results helping to establish preferred experimental and technical strategies for metagenomic analysis studies.