DIFFERENTIAL EFFECTS OF DABIGATRAN ETEXILATE AND TICAGRELOR ON BLEEDING AS ASSESSED BY WASHED BLOOD AND SHED BLOOD TESTS IN HEALTHY SUBJECTS

methods: Washed blood and shed blood methods were tested in two groups of 12 HVs. DE (Pradaxa®, 220 mg, n=8) or ticagrelor (Brilinta®180 mg, n=4) was given as a single dose and bleeding methods were done at 0, 2, (3 only washed blood) and 6 hr. Washed blood: a forearm skin incision with junior Surgicutt® was made and the wound area was washed with saline. Wash solution was collected and measured photometrically. AUC was recorded as blood loss, bleeding time as the irst well with optical density (OD) < 0.05. Shed blood: two incisions in the forearm using an adult Surgicutt® were made and blood emerging from the wound was collected over 4 min and placed in a protease inhibitor solution. Plasma was assayed by ELISA for s-thromboglobulin (s-TG), thrombin-antithrombin (TAT), prothrombin fragment (F1+2), ibrinopeptide A (FPA). Venous blood was taken at the same time points.