Micro-column liquid chromatography with multi-wave-length photometric detection : I. The OB-4 micro-column liquid chromatograph

Abstract The OB-4 micro-column liquid chromatograph is designed for high-performance liquid chromatography (HPLC) on 50–200 μl columns with typical eluent volumes of 500–2500 μl. The detector is a double-beam microspectrophotometer with two flat-window flow cells. The monochromator of the detector contains a special drive for rapid chnge of wavelength according to stepwise cyclic programmes over the range 190–360 nm. In this way multi-wavelength detection on a time-share basis is performed. The OB-4 chromatograph may be used for all types of HPLC. It consumes much smaller amounts of eluents, adsorbents and samples than other instruments. The multi-wavelength facility helps in identification and evaluation of homogeneity. Examples of applications are given.