ABiocatalytically Active Membrane Obtained from Immobilizationof 2‑Deoxy‑d‑ribose-5-phosphate Aldolaseon a Porous Support
2019
Aldol
reactions play an important role in organic synthesis, as
they belong to the class of highly beneficial C–C-linking reactions.
Aldol-type reactions can be efficiently and stereoselectively catalyzed
by the enzyme 2-deoxy-d-ribose-5-phosphate aldolase (DERA)
to gain key intermediates for pharmaceuticals such as atorvastatin.
The immobilization of DERA would open the opportunity for a continuous
operation mode which gives access to an efficient, large-scale production
of respective organic intermediates. In this contribution, we synthesize
and utilize DERA/polymer conjugates for the generation and fixation
of a DERA bearing thin film on a polymeric membrane support. The conjugation
strongly increases the tolerance of the enzyme toward the industrial
relevant substrate acetaldehyde while UV-cross-linkable groups along
the conjugated polymer chains provide the opportunity for covalent
binding to the support. First, we provide a thorough characterization
of the conjugates followed by immobilization tests on representative,
nonporous cycloolefinic copolymer supports. Finally, immobilization
on the target supports constituted of polyacrylonitrile (PAN) membranes
is performed, and the resulting enzymatically active membranes are
implemented in a simple membrane module setup for the first assessment
of biocatalytic performance in the continuous operation mode using
the combination hexanal/acetaldehyde as the substrate.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
44
References
5
Citations
NaN
KQI