Rapid quantitative analysis of multi-components in Andrographis paniculata using UPLC-QqQLIT-MS/MS: Application to soil sodicity and organic farming

Abstract An ultra performance liquid chromatography-triple quadrupole-linear ion trap mass spectrometry method in multiple-reaction monitoring mode was developed for the rapid determination of 12 bioactive compounds in the leaf and stem of Andrographis paniculata ( A. paniculata ). Different soil conditions i.e., soil sodicity and use of organic manure was manually prepared and its effect on the extractive yield and quantity of bioactive phytoconstituents was studied. A good linear regression relationship ( r 2 , 0.9988–0.9999), intra-day precision (RSD, 0.17–3.22%), inter-day precision (RSD, 0.31–3.44%), stability (RSD, 1.19–3.09%), and recovery (RSD, 1.03–3.20%) were obtained for all the analytes. The results showed significant differences in the content of diterpenoids, flavonoids, phenolics and triterpenoid compounds. Major markers i.e., andrographolide and neoandrographolide (187.5 mg/g and 109.0 mg/g, respectively) were detected highest in silty clay loam soil with addition of 3.5% organic manure in leaf of A. paniculata . This study was designed to benefit the cultivators by suggesting them about the suitability of soil and manure conditions to enhance the quantity of major bioactive compounds from A. paniculata . The present work described a highly specific, sensitive and rapid determination method of 12 bioactive compounds.