HUMAN OSTEOBLAST-LIKE MG 63 CELLS IN CULTURES ON NANOFIBROUS PLGA MEMBRANES LOADED WITH NANODIAMONDS

Using an electrospinning technique, we constructed composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA, ratio 85:15) and 33% wt.% of nanodiamond particles. The number of initially adhering human osteoblast-like MG 63 cells on day 1 after seeding, their spreading and subsequent growth were similar on both types of membranes. However, higher cell numbers on day 3 and 7 after seeding and a larger cell spreading area were found in the cells in the control polystyrene cell culture dishes. Nevertheless, the composite PLGA-ND membranes provided relatively good support for colonization with bone-derived cells; thus this material is promising for bone tissue engineering.