Determination of γ-aminobutyric acid in Pinellia ternate (Thunb.) Breit. at different growth stages.

[Objective] This study was to optimize the extraction process of total flavonoids from FRUCTUS LIGUSTRI LUCIDI using ethanol reflux method.[Method] On the basis of single factor test,orthogonal test L9(34) was designed to optimize the parameters related.[Result] The optimized extraction parameters were as follows:2.50 g dried powder of FRUCTUS LIGUSTRI LUCIDI as raw material,60% ethanol as extractant,solid-liquid ratio of 1:40,extraction temperature of 85 ℃,extraction duration of 2 h.Using these parameters the extraction yield of total flavonoids was as high as 6.215%.[Conclusion] Ethanol reflux method is stable and reliable in the extraction of total flavonoids from FRUCTUS LIGUSTRI LUCIDI.