Bioinformatics Analysis on the Structure and Function of Malate Dehydrogenase Gene of Taenia solium

Objective: To analyze and predict the structure and characteristics of Taenia solium malate dehydrogenase(MDH),and so as to guide the experimental research on biological function of MDH.Methods: Tools about informatics analyis on sequences and structures of gene and protein in protein analysis expert system of bioinformatic institute of Switzerland,and those of state biological and technology information center of USA,combined with Pcgene and Vector NTI suite bioinformatics software pakege were employed to screen Taenia solium MDH gene and encoding region from cDNA plasmid library to analyze and predict physicochemical properties of its encoding protein,modification site after translation,function domains,subcelluar location,topological structure,secondary structure,and 3D conformation and so on.Results: This gene encoded 332 amino acids,and was a full length gene.It was the most homologues to Taenia echinococcus MDH in GenBank.Its molecular weight in theory was 36 459.2 Da.Its encoding protein was predicted to have no transmembrane region or disulfide bonds,and was stable.Its evolutionary reltionship was closest to fasciola genus MDH.Conclusions: The cDNA sequence encoding MDH is screened from cDNA library of adult Taenia solium by bioinformatical method.The information about structure and function of Taenia solium MDH gene and protein were obtained.