Potentiation of 5-lipoxygenase metabolism by human neutrophils using a combination of activators

Abstract Platelet-derived 12-hydroperoxy-5,8,10,14-icosatetraenoic acid (12-HPETE) cannot increase the synthesis of leukotriene B4 metabolites by human neutrophils activated by 1-0-hexadecyl-2-acetyl-sn-glycero-3 phosphocholine (Paf Acether) at 10 -9 and 10 -8 M in contrast to 1 μM arachidonic acid. The combination of the 3 inducers at various concentrations resulted in a potentiation of the formation of leukotriene B 4 metabolites suggesting that multiregulatory steps play a key role to govern the 5-lipoxygenase activity. These results suggest that the use of agents, that can act synergistically to produce leukotrienes may be worthwhile investigating to understand some aspects of the leukotriene biosynthesis.