Analysis on genetic diversity of different Salvia miltiorrhiza geographical populations in China

OBJECTIVE: To research on genetic diversity of different Salvia miltiorrhiza geographical populations in China. METHOD: The genetic diversity of 27 S. miltiorrhiza geographical populations from ten provinces in China was estimated using amplified fragment length polymorphism (AFLP) markers. The data of amplified bands were analyzed by the software POPGENE and SPSS. RESULT: The ten primers employed produced a total of 528 discernable and reproduceable amplified fragments. There were 476 polymorphic brands. The percentage of polymorphic bands with in different populations was 90.15%. Genetic diversity analysis showed that Neis gene diversity (He) was 0.261 2 and Shannon's genetic diversity index (1) was 0.403 3. The coefficient of gene similarity was 0.504 0-0.789 0 between populations. The cluster map including all samples were obtained by UPGMA. In the map, there were seven cluster groups and one individual outside the groups. CONCLUSION: The genetic diversity with in different geographical population of S. miltiorrhiza in China is plentiful.