Synthesis of PEO-based di-block glycopolymers at various pendant spacer lengths of glucose moiety and their in-vitro biocompatibility with MC3T3 osteoblast cells

AbstractDi-block copolymers of poly(ethylene oxide) (PEO) and glycopolymers at three different pendant spacer lengths of glucose moiety were synthesized by deacetylation of pendant moieties of 2,3,4,6-tetra-O-acetyl-d-glucopyranosides which were prepared by reversible addition-fragmentation chain-transfer (RAFT) process. The water soluble deacetylated copolymers, PEO-b-poly(acryl-d-glucopyranoside) (PEO-b-PAG, 2a), PEO-b-poly[4′-(acryloxy)butyl-d-glucopyranoside] (PEO-b-PABG, 2b), and PEO-b-poly[6′-(acryloxy)hexyl-d-glucopyranoside] (PEO-b-PAHG, 2c) were characterized in comparison with acetylated copolymers by spectroscopic and gel permeation chromatography methods. In order to assess the biocompatibility of these three di-block copolymers, the adhesion (%), viability (%), and proliferation assays were carried out in MC3T3 cells in-vitro at different concentrations from 10 nM to 1000 μM. Qualitative analysis of cell cytoskeletal organization was obtained by immunostaining with talin and integrin α5. It w...