Cephaleuros virescens in Brazilian Mahogany: Algae Parasitic Disease Threatening an Important Reforestation Tree
2020
The forest species are frequent targets of diseases. In this context, the plant-parasitic algae comprise a separate group in this process, including the algae genus Cephaleuros , which attacks mahogany cultivars. The objective of this work was to accomplish the molecular and morphological characterization of the causal agent of algae spots occurring in Brazilian mahogany ( Swietenia macrophylla ). Somatic and reproductive algae structures contained in lesions on the leaves had the DNA extracted and amplified by primers 18SHf and 18SLr of the rRNA nuclear gene 18S. Then, the amplicons were purified and sequenced. The algae structures too were characterized under light microscope. In addition, a total of 12 injured leaves located in the lower third of each attacked tree were randomly extracted for lesion characterization according to its size and number. After algae structures measurements, sporangiophores and sporangia had 260.2 x 12.9 μm and 25 x 18.8 μm. These measurements, combined with the molecular identification, indicated that the algae found in Brazilian mahogany leaves is C. virescens . Attacked leaves showed an average of 33 lesions, 60% of which were smaller than 2 mm in diameter, which shows great capacity of the pathogen to reach different parts of the foliar limb. These measurements, combined with molecular identification, confirmed the algae found in Brazilian mahogany leaves to be C. virescens .
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