Rapid automated determination of d-penicillamine in plasma and urine by ion-exchange high-performance liquid chromatography with electrochemical detection using a gold electrode

Abstract A high-performance liquid chromatographic method for the determination of d -penicillamine in plasma and urine has been developed, based on separation on a cation-exchange resin followed by an amperometric detection of the SH group of d -penicillamine oxidized on a gold electrode. The method has been automated and separations from plasma and urine take 7 and 9 min, respectively. The detection limits are 0.05 μg/ml in plasma and 0.2 μg/ml in urine, with a coefficient of variation of 2.9% ( n = 10).