Clinical Value of SARS-CoV-2 Nucleic Acid Test in Different Populations/ 不同人群新型冠状病毒核酸检测的临床价值

Objective: To explore the application value of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid test in different population, including persons in fever clinic and isolation wards, susceptible persons as well as persons in large-scale screening, provide reference for laboratory nucleic acid test of SARS-CoV-2 Methods: Laryngeal swabs from different population in People's Hospital of Zhengzhou between February 1, 2020and March 22, 2020 were collected, including patients in fever clinic and isolation wards, anti-epidemic frontline health care workers (HCWs) , hospitalized patients without symptoms of coronavirus disease 2019 (COVID-19),accompanying family members, and persons returning to work Real- time reverse transcriptase polymerase chain reaction (RT-PCR) and two domestic detection kits A and B were used to test SARS-CoV-2 nucleic acid in patients in fever clinic and isolation wards Results: A total of 15 497 laryngeal swabs were detected, 24 of which were positive for SARS-CoV-2 nucleic acid test, nucleic acid test positive cases were all from fever clinic and isolation wards 24cases of SARS-CoV-2 nucleic acid test positive laryngeal swabs were all negative for blood specimens tested at the same time The first laryngeal swab test results of kits A and B were identical, re-test of 2 confirmed cases aftertreatment were positive in kit A group and negative in kit B group Nucleic acid test results were all negative in frontline HCWs, hospitalized patients without COVID- 19 symptoms, accompanying family members and physical examination persons returning to work Conclusion: No positive results are found in the screening among the returning workers, hospitalized patients, accompanying family members and front- line epidemic prevention and control professionals, positive rate of SARS- -CoV-2 nucleic acid of laryngeal swab specimens is higher than that of blood specimens, positive rates of different nucleic acid test reagents are slightly different