Enantioselective Q uantification o f A tenolol i n M ouse P lasma b y H igh P erformance L iquid C hromatography U sing a C hiral S tationary P hase: A pplication t o a P harmacokinetic S tudy

Enantiomeric resolution of atenolol was achieved on the HPLC vancomycin macrocyclic antibiotic chiral stationary phase Chirobiotic V. The polar ionic mobile phase consisted of methanol–glacial acetic acid– triethylamine (100 + 0.025 + 0.75, v/v/v) at a flow rate of 0.8 m L/min. Fluorescence detection at 275/305 n m for excitation and emission, respectively, was used. Plasma samples were purified using SPE on Oasis HLB cartridges. The calibration curves in plasma were linear over the range of 5–400 n g/mL (r = 0 .999) for each enantiomer with an LOD of 1.0 n g/mL. The proposed method was validated in compliance with International Conference of Harmonization guidelines in terms of linearity, accuracy, precision, LOD, LOQ, and selectivity. The overall recoveries for S-(−)- and R-(+)-atenolol enantiomers from plasma were 95.0–99.5%; RSD ranged from 2.5 to 3.3%. The developed method was applied for the trace analysis of atenolol enantiomers in plasma and for the pharmacokinetic investigation of atenolol enantiomers in mouse plasma.