Idebenone Treatment Mediates the Effect of Menadione Oxidative Stress Damage in Saccharomyces cerevisiae
2012
We investigated the damage caused by oxidative stress using the yeast Saccharomyces cerevisiae as a
model biological system. After inducing oxidative stress with menadione, we were able to evaluate the extent of
cellular oxidative stress by utilizing 2´,7´-dichlorofluorescein diacetate (DCFH-DA) as a marker of the presence of
reactive oxygen species. Cells were grown on different carbon sources in order to compare fermentative and oxidative
metabolism. Under these conditions we evaluated the effectiveness of idebenone (2,3-dimethoxy-5-methyl-6-(10-
hydroxydecyl)-1,4-benzoquinone) as a molecule that could relieve menadione-induced growth inhibition in Saccharomyces
cerevisiae.
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