ИЗУЧЕНИЕ ЧАСТОТЫ АДАПТИВНЫХ МУТАНТОВ И ГЕТЕРОГЕННОСТИ ПОПУЛЯЦИИ КЛЕТОК ШТАММОВ PSEUDOMONAS AERUGINOSA ATCC 27853 И С ФЕНОТИПОМ МЕЛКИХ КОЛОНИЙ В РЕЗУЛЬТАТЕ ДЕЙСТВИЯ ЦИПРОФЛОКСАЦИНА

This paper presents a comparative analysis of the frequency of adaptive mutants to ciprofl oxacin in the cultures of planktonic and biofi lm cells of the reference strain of Pseudomonas aeruginosa ATCC 27853 and strain with a phenotype of small colonies. The experiments detected direct dependence on the frequency of adaptive mutants growth phase culture of a strain with a phenotype small colony varyants in culture and the biofi lm – from the time of incubation with the antibiotic. Cell survival biofi lms under the action of ciprofl oxacin was higher compared to cell survival planktonic cultures of both strains. It was demonstrated the high heterogeneity of strain having small colony variant after incubation with the antibiotic, despite its high sensitivity to ciprofl oxacin. With increasing time of incubation with ciprofl oxacin for the cells with the phenotype of a strain of small colonies predicted the possible emergence of more resistant to the antibiotic clones.