[Study on corelation between InsP(3) generation and TCR/ CD3 complex-mediated [Ca(2+)]i responses in T cells from SLE patients].

AIM: To demonstrate whether function disorder of T cells from SLE patients was relative to abnormal biochemical pathways mediated by TCR/ CD3 complex and whether this abnormality was relative to the InsP 3 production. METHODS: Human T cells were isolated through Nylon columns from heparinized peripheral blood from SLE patients and control individuals. Percentage of CD3 + T cells was detected by flow cytometry. After cross linking of anti CD3 mAbs to sheep anti mouse IgG and stimulating T cells, the changes of free calcium ion within T cells was observed successively for 10 minutes by an adhesion cytometry. Flow cytometry was used to detect the difference in positive rate between normal individual’s and SLE patein’s T cells. Level of InsP 3 was detected by a radioreceptor assay kit. RESULTS: ①Flow cytometry analysis showed that CD3 + T cells obtained through nylon column accounted for more than 90%. ②The base line recordings of \[Ca 2+ \]i response from SLE patients were similar to that from normal control ( P =0.105). Peak and plateau \[Ca 2+ \]i response in T cells of SLE patients were significantly higher than that in the control group ( P 0.001 ). ③The percentage of the CD3 + T cells was similar in both individuals( P =0.665). ④No differences in the anti CD3 mAb mediated InsP 3 generation were found between the two groups ( P =0.537). CONCLUSION: TCR/ CD3 mediated \[Ca 2+ \]i responses in T cells from SLE patients is abnormal, and the abnormality has no relation to InsP 3 generation.