Impact of PAHs on the expression of PRDX in earthworm (Eisenia fetida)

A peroxiredoxin6(PRDX6)-like gene segment has been found in up-regulated cDNA libraries of earthworm Eisenia fetida exposed to benzo[a]pyrene(BaP).Analysis with basic local alignment search tool(BLAST) and phylogenetic analysis revealed that the gene segment contains a characteristic motif which was encoded by Cys,illustrating that this gene segment belongs to PRDX.To verify the response of PRDX in E.feitida exposed to poly aromatic hydrocarbons(PAHs),four-week pollution experiments were conducted following the methods recommended by the Organization for Economic Co-operation and Development(OECD).The results showed that the expression of PRDX was up-regulated in earthworm exposed to 1.0 mg·kg-1 Pyrene and BaP.It was indicated that PRDX in E.fetida was the potential molecular biomarkers of antioxidative stress caused by soil contamination.