High-affinity l-arabinose transport operon: Gene product expression and mRNAs☆

Abstract Various portions of the “high-affinity” l -arabinose transport operon were cloned into the plasmid expression vector pKK223-3 and the operon-encoded protein products were identified. The results indicate that three proteins are encoded by this operon. The first is a 33,000 M r protein that is the product of the promoter-proximal l -arabinose binding protein coding sequence, araF . A 52,000 M r protein is encoded by sequence 3′ to araF and has been assigned to the araG locus. The sequence 3′ to araG encodes a 31,000 M r protein that has been assigned to the araH locus. Both the araG and araH gene products are localized in the membrane fraction of the cell, implying a role in the membrane-associated complex of the high-affinity l -arabinose transport system. Nuclease S 1 protection studies indicate that two operon message populations are present in the cell, a full-length operon transcript and a seven- to tenfold more abundant binding protein-specific message. The relative abundance of these two message populations correlates with the differential expression of the binding protein and the membrane-associated proteins of the transport system.