Method for detecting taurine in functional beer by adopting high performance liquid chromatography (HPLC)

HPLC detection method using one kind of functional taurine beer, comprising the steps of :( a) is derived pretreatment procedure: 6-quinolinyl -N- hydroxysuccinimide urethane pre-column derivatization imido ; (b) gradient elution program: mobile phase a was 139.92mmol / L sodium acetate trihydrate + 16.39mmol / L triethylamine buffer, with 50% phosphoric acid to adjust the pH to 4.98, respectively, optimized for beer samples and standards gradient elution program, so taurine beer other interfering substances are completely separated, the column of the sample analysis using the analytical C18 column was Waters AccQ-Tag amino U.S. company, 3.9mm * 50mm, 4um; (c) data processing procedure: the test beer and taurine standard spectra comparison, retention time qualitative, quantitative chromatographic peak area. The present invention is implemented in beer taurine completely separated from other amino acids, so that its precise qualitative and quantitative. The method is simple, accurate and reliable, has high sensitivity.