Occurrence and susceptibility of hospital strains producing enzymes with a wide spectrum of substrate profiles

: In connection with more and more frequent occurrence of extended spectrum beta-lactamases an effort has been made to detect their presence in the biological materials coming from the patients of different wards of Mining Hospital in Sosnowiec from January to September 1995. Among 38 diagnostic materials, from which the strains in question were isolated, the following materials have been examined: bronchial lavage (52.6%), surgical wounds (18.4%), urines (15.8%) and others (13.2%). The examined diagnostic materials came from Intensive Care Unit (87.0%), Casualty and Orthopaedic Surgery Clinic (5.0%) and General Surgery Clinic (3.0%). The most frequently isolated microorganisms were Klebsiella pneumoniae. (47.4%), Serratia marcescens (36.8%), Enterobacter cloacae (10.5%) and others (5.3%). Half of the examined strains constituted those producing extended spectrum beta-lactamases and probably possessing an extra resistance mechanism: (Serratia marcescens--74%, Enterobacter cloacae--21%, Enterobacter aerogenes--5.0%) and 50% were those producing extended--spectrum beta-lactamases. (Klebsiella pneumoniae--95%, Escherichia coli--5%). Susceptibility of the examined strains to anti-microbial antibiotics was diverse. Full susceptibility of the analysed strains has been observed exclusively in case of Karbapenems (Imipenem, Meropenem and Cefamycyn). In case of beta-lactamase with beta-lactamase inhibitor combinations susceptibility was diverse and dependent on the species of the examined microorganism.