Expression, purification and identification of simian type-D retrovirus p27 gene.

The simian type-D retrovirus p27 gene was PCR amplified with a pair primers designed according to the published genomic sequence of SRV-2 strain.The PCR fragment was cloned into prokaryotic expression vector Pbad/Thio-TOPO vector and sequenced.The amplified p27 sequence was identical to that of SRV-2 strain.The positive recombinant explasma was tranfered into host cell TOP10 and expressed under Arabinose induction.The expressed recombinant product was identified by SDS- PAGE and Western Blot,and purified by proBond~(?) column under native conditions.The results demonstrated that recombinant protein had a molecular weight of 50 KDa and its size in line with expectation.