Method for measuring high performance liquid chromatography characteristic fingerprint of isatis root and isatis root preparation

The invention discloses a method for measuring a high performance liquid chromatography characteristic fingerprint of isatis root and an isatis root preparation. The method comprises the following steps of: a) pretreating isatis root and isatis root preparation samples to be measured, namely adding water into the samples to be measured, heating, shaking well, filtering and taking subsequent filtrate; and b) measuring the pretreated isatis root and isatis root preparation samples to be measured by a high performance liquid chromatography, and thus obtaining the characteristic fingerprint. By the method, characteristic fingerprints of isatis root, isatis root granules, compound isatis root granules and baphicacanthus root are detected and analyzed under the same high performance liquid chromatography condition for the first time; and the method has the advantages of high repeatability, low analysis cost and the like, is simple, convenient and quick, has guiding significance for quality evaluation and identification of the isatis root and the isatis root preparation, can be applied to research and development, quality control and other aspects for new medicines, and has high practicality.