Expression of Recombinant Potato leafroll virus Structural and Non‐structural Proteins for Antibody Production

Vector pMPM-A4Ω and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp 43 - 616 and RdRp 304-537 were chosen for expression. A high level of CP and RdRp 304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosettagami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.