ANALYZING THE EFFECT OF BETA-BOSWELLIC ACID (BBA) TO DOPAMINERGIC NEURON DIFFERENTIATION OF TRANSGENE EXPRESSING MOUSE EMBRYONIC STEM CELLS

Introduction: Parkinson's disease is a degenerative neurological disorder associated with progressive loss of dopamine-secreting neurons in the direction of the Substantia nigra and the basal ganglia. In the past, drug treatment was the only known solution for the disease. But now it has been tries to use modern medical methods and herbal extracts to reduce the effects of chemical drugs. Given the above, the main aim of the present study was to investigate the effect of beta-boswellic acid (BBA) for dopaminergic production of produce embryonic stem cells expressing the foreign genes using lentivirus vectors in Parkinson's disease. Method: In this study, using the gene transfer techniques based on lentivirus vectors, Nurr1 genes (A transcription factor involved in the development of DAn and GPX1 (An antioxidant enzyme) are transferred to the R1 murine embryonic stem cells and the permanent expression of these genes in these cells is confirmed. For the differentiation of murine embryonic stem cells expressing the nurr1 and gpx1 genes to Dopaminergic neurons with the function the cell experiments are used and the making EB protocol was used to distinguish the embryonic stem cell to dopaminergic neurons. Then at the end of the third, fourth and fifth phases of