Structures and pharmacological activities of beesiosides O and P

AIM To investigate the chemical constituents of the rhizomes of Beesia calthaefolia native to China in order to obtain a more comprehensive understanding of its effective components. METHODS Compounds were isolated by column chromatography with silica gel. Their structures were elucidated by spectral analysis and chemical evidence. Compounds identified were subjected to pharmacological evaluation. RESULTS Two novel compounds were isolated and identified as (20 S ,24 S ) 15α acetoxy 16β,24;20,24 diepoxy 9,19 cyclolanostane 3β,25 diol 3 O β D xylopyranoside (I) and (20 S ,24R) 15α acetoxy 9,19 cyclolanostane 3β,16β,20,24,25 pentaol 3 O β D xylopyranoside (II), named beesioside O and beesioside P. CONCLUSION Compounds I and II are new compounds. Compounds I exhibited immunosuppressive activity and could inhibit angiogenesis as well as inhibit the proliferation of osteoblast. Compound II displayed remarkable inhibition activity against calcium channel receptor.