KH2PO4 AND SUCROSE AFFECTED in vitro AND ex vitro DATE PALM PLANTLETS CV SAKOTY

The acclimatization stage of date palm plantlets is crucial for increasing numbers of plants to culture in the open field so to increasing the survival percentage of this stage in the greenhouse this study was done to improving plantlets in the rooting stage in vitro by using different concentrations of KH2PO4 as full (170 mg L-1), full (170 mg L-1) + ¼ and full (170 mg L-1) + ½ and sucrose 30 as control treatment, 45 and 60 g L-1. The found results exhibited the treatment KH2PO4  at full  with 45 g L-1 sucrose  proved to be the great treatment for  increasing in vitro height plants, while the root length and numbers and stem thickness were highly increasing under KH2PO4 full + ½ with 60 g L-1 sucrose, maximum hairy roots produced by full KH2PO4 +30 g L-1 sucrose, highest numbers of leaves obtained on the medium supplemented by full KH2PO4 + ¼  with 45 g L-1 sucrose, the greatest percentage of plantlets acclimatization under full + ¼ KH2PO4 with 30 and 45 g L-1 sucrose. More contents of total indoles, chlorophyll a and b were present under full +1/4 and full + ½ KH2PO4 with 45 g L-1 sucrose. Up on the previous results the treatment of full + ¼ or full + ½ KH2PO4 with 45 g L-1 sucrose is the preferable for successful in vitro and ex vitro stages.