A particle accelerating device for delivering DNA material into plant cells

A particle acceleration device for implanting DNA material into plant cells using a timed burst of pressurized helium gas was developed. The device did not require the use of consumable materials (other than helium and microparticles), as used in some similar systems, and was thus easier and less expensive to operate. The system was designed to provide more precise control of the duration of the helium burst and of pressure differentials to ensure more reproducible results. Safety interlock circuitry was incorporated. The device was found to be efficient for delivery of DNA into alfalfa and soybean cell cultures.