Study in the killing effect of Myxoma virus to C6 glioma cell in vitro

Objective To evaluate the susceptibility of C6 glioma cells to Myxoma virus and the killing effect of Myxoma virus to the C6 glioma cells in vitro.Methods C6 glioma cells were infected with myxoma virus,used death virus as the negative control,5-FU as the positive control,DEMD as blank control.The number of living cells were counted every 24 h,and Western-Blot method,inverted microscope and MTT assay were applicated to observe the cell morphology and survival rate in each group.Results The cell number were decreased rapidly in virus effected group and 5-FU group,with significant differences to the negative and blank control groups.And cells in virus effected group appeared cytopathic effect.Conclusions C6 glioma cells were susceptible to myxoma virus and myxoma virus had killing effect to C6 glioma cells in vitro. Key words: Glioma; Myxoma virus; Disease susceptibility; in vitro