Effect of triamcinolone acetonide on expression of ICAM-1 and ILK in RPE cells cultured by high glucose

Objective To investigate effect of inflammation on diabetic retinopathy(DR)by detecting expression of intercellular adhesion molecule-1(ICAM-1)and integrin-linked kinase(ILK)in retinal pigment epithelial(RPE)cells induced by high glucose and observing effect of triamcinolone acetonide(TA)on their expression.Methods Human RPE cells were cultured in vitro.Immunofluorescent staining and Western-blot was used to detect expression of ILK and ICAM-1 in PRE cells of high glucose group(DMEM culture medium with 30.0 mmol·L-1 glucose),TA group(DMEM culture medium with 0.1 mmol·L-1 and 30.0 mmol·L-1),respectively.RT-PCR was used to detect expression of mRNA of ILK and ICAM-1.Results Western-blot and immunofluorescent staining showed high expression of RPE cells in high glucose group for culturing 6 hours.It was 0.378±0.012 and 1.71 times to 0.220±0.008 in control group.TA had inhibitive effects on its expression,which was 26.98%(P0.05).Expression of ILK protein was the highest in high glucose group at 2 hours,was 1.45 times to that of control group,and was higher than that in TA group,which was 76.20% of high glucose group.There was no significant difference(P0.05).TA had no obvious inhibition on expression of ILK.RT-PCR showed that expression of ICAM-1 and ILK mRAN was gradually increased in RPE cells in high glucose group at 1 hour,expression of ICAM-1 mRNA went to peak at 2 hours,inhibitive rate of TA on ICAM-1 mRNA was 83.67% at 2 hours,there was statistical difference(P0.05).There was no difference in expression of ILK mRNA between TA group and high glucose group(P0.05).Conclusions There are high expression of ICAM-1 and ILK in RPE cells under high glucose circumstance.Glucocorticoid TA can inhibit up-regulation of ICAM-1 in RPE cells induced by high glucose,while up-regulation of ILK is not inhibited,which indicate that inflammation plays a role in pathology of DR at early stage,and RPE cells may play a part in DR with the signaling pathway of these two factors.