ИССЛЕДОВАНИЕ ИНГИБИРУЮЩЕГО И РАЗРУШАЮЩЕГО ДЕЙСТВИЯ ПРЕПАРАТА НАНОЧАСТИЦ СЕРЕБРА НА БИОПЛЕНКИ, СФОРМИРОВАННЫЕ КЛИНИЧЕСКИ ЗНАЧИМЫМИ МИКРООРГАНИЗМАМИ

AIM: to obtain and investigate the activity of silver nanoparticles stabilized with arabinogalactan in relation to clinically relevant strains of filmforming microorganisms. MATERIALS AND METHODS: silver nanoparticles were obtained by reduction from silver nitrate in the presence of arabinogalactan with additional stabilization with dioctyl sodium sulfosuccinate. The shape and size of the nanoparticles were determined by the method of transmission electron microscopy, the zeta potential by the method of electrophoretic light scattering. The study of the effect of the nanoparticles on biofilm formation was carried out on 17 clinically relevant strains of bacteria isolated from blood culture and the clinical biomaterial of postoperative patients. RESULTS: the silver nanoparticles with an average diameter of 11.4 nm and a zeta potential of –24 mV were obtained. The minimum inhibitory concentration of the nanoparticles in relation to planktonic form of bacteria was 120 µg/ml; the use of the drug at a concentration of 100 µg/ml reduced the amount of CFU by 7 orders of magnitude compared with the initial culture. The study of the effect of silver nanoparticles on the formation of biofilms showed that, in the presence of the drug, the growth of biofilms was significantly reduced; at a drug concentration of 150 µg/ml, the growth of bacterial films was completely suppressed. Incubation of the formed daily biofilms with the silver nanoparticles in the concentration range from 150 to 120 µg/ml for 48 h resulted in the partial or complete destruction of the biopolymer matrix. CONCLUSION: the studied preparation of silver nanoparticles has a great potential for use in the treatment of infectious diseases caused by biofilm forming microorganisms.