Identification of Anchoring Motifs Used for the Cell Surface Display from Outer Membrane Proteomes

In biotechnology, proteomics can have practical applications, for example, through the identification of proteins that may be potential targets for the biotechnology industry, and through the extension of our understanding of the physiological action of these proteins. In this study, outer membrane proteomes were analyzed and then used to identify potential candidates for applying anchoring motifs. We found a few of proteins that fit specific requirements, such as an efficient signal peptide or transport signal to that allows a premature fusion protein to be transported across the inner membrane and a strong anchoring structure that keeps fusion proteins on the cell surface without detachment. Thus, this study shows proteomics is very important tools for the discovery of targets that have potential biotechnological applications. [This work was supported by the Basic Science Research Program (2010-0008826) and Converging Research Center Program (2009-0093652) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology]