Microbial Diversity of Guantao-3 Member of Zhongyi Block,Gudao Oil Field in China

To develop microbial enhanced oil recovery(MEOR) technology,microbial diversity of Guantao-3 Member of Zhongyi Block,Gudao Oil Field in China after water and chemical flooding was investigated by molecular biology methods. Two samples of injected water and four samples of produced water were analyzed. The results indicated the diverse of microbial communities in the injected water,which was dominated by Gammaproteobacteria and Betaproteobacteria,was more complicated than that in produced water,and the data also showed that there were notable differences of microbial communities in different oil wells,and the microbial community in a single well also changed with the time courses. Besides,the species diversity of bacteria in produced water,which was dominated by Gammaproteobacteria,was relatively simple;while that of archaea was relatively rich,including Methanobacteria,Methanomicrobia,Archaeoglobi and Thermococci. These results provide the basis information on microbial ecology in specific reservoirs for MEOR technology.