[The initiation of G2/M checkpoint by diallyl disulfide requires the activation of p38 MAP kinase in HL-60 cells].

Objective To explore the molecular mechanisms of G 2/M checkpoint initiated by diallyl disulfide(DADS) in HL 60 cells. Methods Cell viability was determined by MTT assay. Cell cycle was assayed by flow cytometry. The expression of phospho p38, Cdc25B and Cdc2, and p38 mRNA were measured by Western blotting and RT PCR, respectively. Results After treatment with DADS at 5 ～160 μmol/L for 0～72 h, the growth of HL 60 cells were suppressed in a concentration dependent manner and the inhibitory effect of DADS(20 μmol/L) was similar to that of ATRA(10 nmol/L) (P0.05). Incubation of HL 60 cells with DADS(20 μmol/L) for 12 h could activate G 2/M checkpoint and increase the expression of phospho p38 MAPK, followed by the expression of phospho Cdc25B and phospho Cdc2(P0.05). SB202190, a specific inhibitor of p38 MAPK, markedly blocked the phosphorylation of p38 MAPK , Cdc25B and Cdc2(P0.05). Conclusion DADS could induce the G 2/M arrest in HL 60 cells which may be involved in the activation of p38 MAP kinase.