Purification Strategies for Microbial Pectinases

Microbial pectinases currently occupy a place of prominence among biocatalysts owing to their capability to catalyze a diversity of reactions in aqueous and non-aqueous media. Specific action of these enzymes has initiated remarkable attention among industrialists and researchers. Pectinases from a large number of fungal and a few bacterial sources have been purified to homogeneity. This has assisted in successful sequence and three dimensional structure determination leading to a better understanding of their unique structural function relationships during various hydrolytic and synthetic reactions. Since protein purification is normally done in a series of sequential steps involving a mixture of different techniques. This article presents a critical review of different approaches which have been employed for the purification of fungal and bacterial pectinases. This will be immense help for researchers while planning microbial pectinase purification.