Methodologies for cloning and expression of immunodominant protein LipL32 of pathogenic Leptospira

Pathogenic serovars of Leptospira have wide antigenic diversity, which is mainly attributed to the lipopolysaccharide which is present in the outer membrane. The most common surface exposed immunodominant lipopolysaccharide protein is LipL32. In the present study the highly conserved surface exposed LipL32 protein was cloned and expressed in pRSET B vector. The His-tagged protein was purified and characterized by western blot analysis. The developed LiPL32 recombinant protein will serve as a novel diagnostic candidate for effective diagnosis of pathogenic forms of Leptospira in animals.