Research on growth state and physiological changes of Epimedium wushanense under different shade conditions

Objective: To study the growth state and physiological changes of Epimedium wushanense under different light conditions(100%,80%,60%,40%,20%),and investigate its adaptation mechanism to shade environment so that it can provide theoretical basis for cultivation and production.Method: The growth index,the content of photosynthetic pigments,proline content,MDA content,soluble sugar content,soluble protein content and antioxidant enzymy activity of E.wushanense under different shade conditions were measured.Result: Except for length/width,the other form index of E.wushanense changed significantly.The E.wushanense grew well between light intensity 80%-40%,besides,the biomass above the earth was relatively high.The chlorophyll a,chlorophyll b,total chlorophyll content and carotenoids cotent were enhanced after shaded,the values of chlorophyll a\chlorophyll b increased between light intensity 100%-60% and reduced between light intensity 60%-20%.The proline and MDA content decreased after shading,the soluble sugar and soluble protein content increased respectively between light intensity 100%-60%,100%-40% and reduced respectively between light intensity 60%-20%,40%-20%.The SOD activity,CAT activity decreased after shading,and the POD activity,APX activity reduced between light intensity 60%-40%,raised under 20% treatment.Conclusion: 80%-40% irradiance treatment was favorable to the growth of E.wushanense.