M1211 Anti-Inflammatory Effect of Novel Lactobacillus Isolated from Japanese “Funazushi” On DSS-Induced Colitis

BACKGROUND: Genus Lactobacillus is classified broadly into two categories. One is isolated from dairy products or gut and another is vegetable -derived Lactobacillus (VDL) isolated from fermented vegetable food such as pickles or a soy-sauce. Genus Lactobacillus isolated from dairy products or gut have been used for clinical trials in inflammatory bowel disease (IBD) as probiotics, however, VDL was poorly investigated. In this study, we newly isolated two VDL from Funazushi (Japanese old-style sushi; salted and fermented with a rice and a crusian carp) and examined its anti-inflammatory effect on dextran sodium sulfate (DSS)induced colitis. MATERIALS & METHODS: The surrounding rice material of Funazushi, was homogenized and plated on MRS medium for selection of Genus Lactobacillus. A single colony was picked and sorted out by its ability for suppression of proinflammatory cytokines at mRNA level in RAW264.7 cells after LPS stimulation (In Vitro screening). Intragastric administration of Lactobacillus isolated from Funazushi or L.gasseri (ATCC 33323) was performed in 8-week olds-female C57BL/6 mice every day during administration of 6% DSS in water for 7 days. The degree of colonic inflammation and mRNA levels of expression of cytokines in colonic mucosa were determined. Results: Among 500 screened colonies, two different anti-inflammatory types of Lactobacillus were isolated from Funazushi (s193 and s292). Thus, in addition to each Lactobacillus alone administration, we performed the combinatory administration with equivalent amount of s193 and s292 (dual administration). Although each Lactobacillus from Funazushi showed an attenuating effect on inflammation, dual administration significantly improved survival rate, body weight loss and histologic damage score in mice compared with either DSS-treated control, L.gasseri, s193 or s292 alone group. mRNA level of IL-6 was attenuated by s193 alone, and IL-12p40 or IL-23p19 level was decreased by s292 alone. mRNA levels of IL-1β and TNF-α were also remarkably inhibited by dual administration. Conclusions: Two strains of newly established Lactobacillus isolated from Funazushi showed a different anti-inflamattory effect on DSS-induced colitis and that effect was enhanced by simultaneous dual administration. These data suggested that VDL isolated from Funazushi may be useful for treatment for IBD with stronger antiinflammatory effects than Lactobacillus isolated from dairy products or gut.