Effect of ginkgolide B on mitochondria function of SH-SY5Y cells incubated with high glucose

Objective To investigate the effects of ginkgolide B (GB) on mitochondria function of SH-SY5Y cells incubated with high glucose.Methods SH-SY5Y cells were pre-treated with different concentrations of GB or placebo in vitro,prior to administration of high concentration glucose in order to inducing apoptosis.Cell apoptosis was investigated with flow cytometry.In addition,mitochondrial membrane potential,mitochondrially generated reactive oxygen species (ROS),mitochondrial complexes Ⅰ and Ⅲ activity were studied,in order to explore the molecular mechanism of GB.Results GB decreased apoptotic cells (P＜ 0.01),improved the activity of mitochondria complex Ⅰ [(69.2±2.7) vs (51.6±2.9)] (P＜0.01) and Ⅲ [(32.3±2.4) vs (26.2±2.5)] (P＜0.05),reduced mitochondrially generated ROS production (P＜0.01),relieved mitochondrial potential depolarization induced by high glucose [(5.8±0.6) vs (3.1±0.6) (P＜0.01).Conclusions GB may decrease cell apoptosis induced by high glucose by reliving mitochondria malfunction related to ROS. Key words: Ginkgolide B;  Mitochondria;  Reactive oxygen species