Effect of non-homogenous thermal stress during sub-lethal photodynamic antimicrobial chemotherapy
2012
Pathogens could be inactivated via a light source coupled with a photosensitizing agent in photodynamic
antimicrobial chemotherapy (PACT). This project studied the effect of non-homogenous substrate on cell
colony. The non-homogeneity could be controlled by iron oxide nano-particles doping in porous glassy
substrates such that each cell would experience tens of hot spots when illuminated with additional light source.
The substrate non-homogeneity was characterized by Atomic Force Microscopy, Transmission Electron
Microscopy and Extended X-Ray Absorption Fine Structure at Brookhaven Synchrotron Light Source.
Microscopy images of cell motion were used to study the motility. Laboratory cell colonies on non-homogenous
substrates exhibit reduced motility similar to those observed with sub-lethal PCAT treatment. Such motility
reduction on non-homogenous substrate is interpreted as the presence of thermal stress. The studied pathogens
included E. coli and Pseudomonas aeruginosa. Non-pathogenic microbes Bacillus subtilis was also studied for
comparison. The results show that sub-lethal PACT could be effective with additional non-homogenous thermal
stress. The use of non-uniform illumination on a homogeneous substrate to create thermal stress in sub-micron
length scale is discussed via light correlation in propagation through random medium. Extension to sub-lethal
PACT application complemented with thermal stress would be an appropriate application.
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