Comparison of colloidal gold assay and immun of luorescence assay in determination of serum cardiac troponin I

Objective To conduct the methodological evaluation on the colloidal gold assay for detecting cardiac troponin I(cTnl) by the comparison of two detection methods and to investigate its clinical application value.Methods Serum cTnI level was determined by the colloidal gold assay in 20 healthy individuals and 20 patients with acute myocardial infarction(AMI) and the detection results were compared with the results detected by the immunofluoresccnce assay.The sensitivity,specificity and availability of the colloidal gold assay for detecting cTnl were systematically studied.Results The colloidal gold assay(Y) had good correlation with the immunofluorescence assay(X)(Y=0.801X-0.174,r=0.980).There was no statistical difference between two methods(P0.05).The sensitivity,specificity and availability were 85.0%,95.0% and 80.3% respectively.The positive likelihood ratio and the negative likelihood ratio were 17.0% and 15.8% respectively.The positive predictive value(PPV) and the negative predictive value(NPV) were 94.4% and 86.4% respectively.Conclusion The colloidal gold method is reliable,available,fairly rapid and easy to perform with high automation degree.cTnl has the high specificity and sensitivity for diagnosing AMI,and lasts for a long time.This method is suitable for routine determinations of serum cTnl levels in clinical laboratory.