ISSR Genetic Diversity of Opium Poppy Germplasms in Different Ecological Zones

In order to reveal the genetic diversity of opium poppy in different ecological zones,based on the molecular markers of 100 inter-simple sequence repeat(ISSR),poppy and relatives from 12 different areas were amplified and analyzed with optimizing conditions for the primers.The results showed that a total of 109 allelic variations were detected,between 9 and 16 loci for each primer.Among them,primer 807 had the most alleles of 16.The value of allelic polymorphism information content(PIC)ranged from 0.77 to 0.92,which the Maximum is 807(0.92)and the minimum is 847(0.77).Genetic similarity among them ranged from-0.452 to 0.981,and these 12 germplasms were classified into 2 groups.The poppy germplasms sources was less correlated with geographic latitude.There was no evident geographic patterns of the poppy germplasm sources happened.The research will provide basis theoretical for scientific and effective utilization poppy germplasms.