Study on the Relationship between Polymorphism of PLIN Gene and Carcass and Meat Quality Traits in Qinchuan Cattle

To reveal the polymorphism of exon3 and exon4 of PLIN gene in Qinchuan beef cattle and evaluate its relationship with production traits.405 Qinchuan cattle with 18-24 months age under identical feeding condition were selected.PCR-SSCP and sequencing method were applied in this study to detect the SNPs of PLIN exon3 and exon4,and analyze the correlation of SNPs with carcass and meat quality traits.T→C mutation at 156 base site in exon3 and C→T mutation at 14 base site in exon4 of this gene were detected.Correlation analysis showed that the index about slaughter weight,carcass weight,backfat thickness and loin muscle area of individual with AB genotype were significant higher than those with AA and BB genotypes(P0.05),and compared to individual with AA genotype was very significantly different(P0.01).This analysis also showed that the carcass length,carcass chest depth and backfat thickness of individual with DE genotype were significant higher than those with EE and DD genotypes(P0.05),and compared to individual with EE and DE genotypes,the tenderness of individual with DD genotype was very significantly different(P0.01).The results indicated that the SNPs of PLIN gene had effect on the capacity of fat accumulate of Qinchuan cattle,it could be a candidate gene for beef modify and directed to the beef cattle breeding.