Hemagglutination by Mixtures of Human γ-Globulin and Heterogenetic Bacterial Antigen

Summary Study on hemagglutination by mixtures of heterogenetic bacterial antigen and corresponding antibodies revealed the following results: 1.Mixtures of antigen and γ-globulin cause agglutination of human erythrocytes that have been treated with either pancreatic protease, trypsin, ficin, bromelin, or papain. 2.Crude extracts of Staphylococcus aureus, Bacillus subtilis, Bacillus cereus , and Listeria monocytogenes are all effective. 3.Serum Fractions II 1, 2 and II 3 are both effective. 4.Serum containing this antibody in comparable titer does not cause hemagglutination in the presence of antigen. 5.Absorption of heterogenetic antibody from γ-globulin considerably reduces the hemagglutinating capacity of γ-globulin in the presence of antigen. 6.This hemagglutination reaction is inhibited by human and animal serum; the serum factor(s) is heat stable at 75°C and only slightly affected at 100°C. Ultrafiltrates of serum are ineffective. Egg yolk, but not egg white, in high dilutions inhibits the reaction. Mixtures of cardiolipin and lecithin or cholesterol are also effective as inhibitors.